Figure 1: The Biacore T100

 

Figure 2: Surface plasmon resonance (SPR) technology detects the change in reflected light angle from the side of the chip opposite of the flow channel according to the mass bound to the surface of the chip.

 

Figure 3: The kinetics of an interaction can be determined from the information in a sensorgram.

 

Biomolecular Interactions with the Biacore T100

The latest addition to the Laboratory for Genomics and Bioinformatics is the Biacore T100. The Biacore T100 uses surface plasmon resonance (SPR) technology, to detect Biomolecular interactions in real time and label-free. SPR technology detects the change in reflected light angle from the side of the chip opposite of the flow channel according to the mass bound to the surface of the chip. The advantages of the Biacore T100 are its sensitivity, temperature controls, improved microfluidics and software, and automation.

Biacore T100 Applications:

• yes/no binding
• affinity
• binding kinetics
• thermodynamics
• multiple binding
• concentration

Molecules for interactions:

• Nucleic acid
• Protein
• Small molecules
• Membranes
• Whole cells or viruses

Sensor Chips:

• CM5 - carboxymethylated dextran matrix
    *Immobilization via -NH2, -SH, -CHO, -OH, or -COOH groups
• NTA - CM5+nitrilotriacetic acid
    *Immobilization of his-tagged molecules
• SA - CM5+streptavidin
    *Immobilization of biotinylated peptides, proteins, nucleic acids or carbohydrates
• HPA - CM5+lypophilic substance
    *Immobilization of model membrane systems

Videos:

• Specificity
• Kinetics: rates of reaction
• Affinity: the strength of binding
• Concentration
• Multiple interactions during complex formation

• Using surface plasmon resonance (SPR)
• Detecting interactions in realtime
• Biacore Flexchip; a new angle on SPR

Contacts:

Micheal Day phone: 405-271-2337 ext. 32559
Dr. Lydgia Jackson phone: 405-271-1201 ext. 3