Bioanalyzer Submissions

PLEASE NOTE: Due to recent events and the continuing concerns posed by potential bioterrorism agents, we have modified our online submission form to require that you provide us with information on the original biological source of any DNA that you will be asking us to obtain sequence from. "Original biological source" refers to the organism from which the DNA was first found in nature (human, HIV, Staphylococcus aureus, etc.). If you simply put a nonspecific term such as "E. coli clone" or something similar, this will not be sufficient. Further, we will not be able to accept DNA sequencing orders for select agents, until our legal responsibilities are clarified. Our current understanding is that, in order to receive select agent DNAs from you for sequencing, we would need to have a select agent permit, which we do not possess. We realize that adding yet another field to fill out on these forms can be tiresome, but this information is necessary to protect our staff against potential legal difficulties. Thank you very much for your understanding.

            - David W. Dyer, Ph.D.

 

 Bioanalyzer Submissions

Preparation of samples:

1. Please submit a minimum of at least 3ul of each sample in an RNase/DNase free 0.6ml eppendorf tube.
2. Dilute RNA to the following recommended sample concentrations:

   Total RNA and mRNA	25-500 ng/ul
   Small RNA (6-150 nt)	1-100 ng/ul (Total RNA)
   	1-20 ng/ul (Enriched small RNA)
   	100-2000 ng/ul (Oligonucleotides)

3. A submission form must be filled out completely and submitted with your samples. An on-line form is in development.
4. An SUR# (OUHSC) or PO# (off-campus) must be given at the time of submission.
5. Samples will be processed within 24hrs and results e-mailed as a .pdf. If you wish to do further analysis such as overlaying multiple samples and comparisons between chips please indicate you want to receive the .xad experimental files on the order form, then download the latest Bioanalyzer B.02.05 software.Agilent Bioanalyzer software download instructions.
6. Pricing is $35.00 per chip. The chips are one use (empty wells can not be rerun). The RNA 6000 Nano chip for analysis of total RNA and mRNA accommodates up to 12 samples. The Small RNA chip has 11 wells for experimental samples.
7. Place samples in the freezer located in BRC room 1106